Low-resolution genome map of the malaria mosquito Anopheles gambiae.
AUTOR(ES)
Zheng, L B
RESUMO
We have microdissected divisions of the Anopheles gambiae polytene chromosomes, digested the DNAs with a restriction enzyme, and PCR-amplified the DNA fragments to generate a set of pooled probes, each corresponding to approximately 2% of the mosquito genome. These divisional probes were shown to have high complexity. Except for those derived from near the centromeres, they hybridize specifically with their chromosomal sites of origin. Thus, they can be used to map cloned DNAs by a dot blot procedure, which is much more convenient than in situ hybridization to polytene chromosomes. We discuss additional potential uses of these probes, such as easier isolation of molecular markers and genes, including those that cross-hybridize with clones available from other insects. It is expected that the probes will substantially accelerate molecular genetic analysis of this most important malaria vector.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=53099Documentos Relacionados
- Pantropic retroviral vectors integrate and express in cells of the malaria mosquito, Anopheles gambiae.
- Chromosome end elongation by recombination in the mosquito Anopheles gambiae.
- An Integrated Genetic Map of the African Human Malaria Vector Mosquito, Anopheles Gambiae
- A systematic study of low-resolution recognition in protein–protein complexes
- Physical Map of the Malaria Vector Anopheles Gambiae