Low-pH Activation of Bacillus cereus Spores

AUTOR(ES)

Issahary, Gabriela

RESUMO

Heat activation of bacterial spores at low pH was investigated in detail. Unlike activation of spores in distilled water at a neutral pH, activation at low pH involves two superimposed processes: enhanced activation and death. Low-pH-activated spores failed to germinate in d-alanine, in contrast to spores activated at neutral pH, owing to the abolition of alanine-racemase activity. Morphological and permeability changes such as release and partial disruption of spores were dipicolinic acid-observed during low-pH activation. The kinetics pattern of low-pH activation, as well as the change in properties of the spores thereafter, suggest that the mechanism of low-pH activation differs from that of other kinds of heat-activation.

Documentos Relacionados

• Entry of poliovirus into cells does not require a low-pH step.
• Enhanced primary isolation of Legionella pneumophila from clinical specimens by low-pH treatment.
• Low-pH conformational changes of rabies virus glycoprotein and their role in membrane fusion.
• Minjingu phosphate rock availability in low-pH highly weathered soil as affected by added salts
• Receptor Priming of Major Group Human Rhinoviruses for Uncoating and Entry at Mild Low-pH Environments