Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil
AUTOR(ES)
Souza, Fernanda Gil de, Silva, Francini Pereira da, Staggemeier, Rodrigo, Rigotto, Caroline, Spilki, Fernando Rosado
FONTE
Rev. Inst. Med. trop. S. Paulo
DATA DE PUBLICAÇÃO
08/11/2018
RESUMO
ABSTRACT Hepatitis A virus (HAV), a member of Picornaviridae family, is the main causative agent of acute viral hepatitis in the world, mainly in developing countries. HAV may be present in contaminated water and food and its presence is often associated to a lesser extent with socioeconomic factors and environmental quality. The main goals in the present study were to standardize a cell culture combined to a polymerase chain reaction protocol for the detection and quantification of viral viability and analyze whether the virus could be found in water samples collected in four urban streams of Sinos River watershed. Virus recovery was assayed from known virus concentrations measured in experimentally contaminated raw and ultrapure water (MilliQ®). Recovery rates ranged from 270% in raw water to 15,000% in ultrapure water. In a second step, a qPCR coupled to a previous passage in cells, demonstrated more analytical sensitivity when compared to samples assayed without a previous passage in cell cultures. HAV genome was detected in only 1 of 84 samples analyzed, pointing to a very low occurrence of HAV in water samples in the studied region. These findings are remarkable, since no more than 5% of the domestic sewage in this area is treated pointing to a low occurrence of HAV in the population living nearby during the study period.
Documentos Relacionados
- Low occurrence of occult hepatitis B virus infection and high frequency of hepatitis C virus genotype 3 in hepatocellular carcinoma in Brazil
- Low prevalence of human immunodeficiency virus and hepatitis C virus co-infection in a medium size city in southern Brazil
- Sandflies (Diptera: Psychodidae) in rural and urban environments in an endemic area of cutaneous leishmaniasis in southern Brazil
- Surveillance of Giardia and Cryptosporidium in sewage from an urban area in Brazil
- Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil