Low-level laser therapy modulates musculoskeletal loss in a skin burn model in rats
AUTOR(ES)
Martins, Francielle, Rennó, Ana Cláudia Muniz, Oliveira, Flávia de, Minatel, Natália Peruchi, Bortolin, Jeferson André, Quintana, Hananiah Tardivo, Aveiro, Mariana Chaves
FONTE
Acta Cir. Bras.
DATA DE PUBLICAÇÃO
2015-02
RESUMO
PURPOSE: To investigate the effectiveness of low-level laser therapy (LLLT) on gastrocnemius muscle morphology and Myod imunoexpression in a model of dorsal burn in rats. METHODS: Sixteen male Wistar rats were distributed into two groups: control group (CG): rats submitted to scald burn injury without treatment and laser treated group (LG): rats submitted to scald burn injury and treated with laser therapy. Fourteen days post-surgery, gastrocnemius muscle was evaluated being the specimens stained with HE and morphometric data was evaluated. MyoD expression was assessed by immunohistochemistry. RESULTS: The results showed that laser treated animals presented more organized tissue morphology compared to the non-treated animals, with a higher number of nucleus in the fibers. Also, the cross sectional area of the fibers and the MyoD immunoexpression in the laser treated groups was higher. CONCLUSION: Low-level laser therapy had positive effects on gastrocnemius muscle, improving tissue muscle morphology, increasing cross sectional area and MyoD immunoexpression.
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