Loss of proliferative calcium dependence: simple in vitro indicator of tumorigenicity.
AUTOR(ES)
Swierenga, S H
RESUMO
The proliferative activities of three lines of "normal" epithelioid rat liver cells and six tumorigenic liver cell lines in the presence of a wide range of calcium concentrations were measured by a simple colony forming assay. The proliferative activities of the normal cells and, to a lesser extent, of the cells of a marginally tumorigenic line were directly proportional to the extracellular calcium concentration. The proliferative activities of the cells of the strongly tumorigenic lines, on the other hand, were either uninfluenced by or inversely proportional to the extracellular calcium concentration. Thus, the proliferative response to the extracellular calcium concentration is a sensitive indicator of the carcinogenic potential of liver cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=393119Documentos Relacionados
- Rates of aggregation, loss of anchorage dependence, and tumorigenicity of cultured cells.
- Restoration of CD44H expression in colon carcinomas reduces tumorigenicity.
- Involvement of integrin alpha V gene expression in human melanoma tumorigenicity.
- DRUG DEPENDENCE: Aspects of ego functions
- Interrupting the early region of polyoma virus DNA enhances tumorigenicity.