Location of sites of transposon Tn916 insertion in the Mycoplasma mycoides genome.

AUTOR(ES)

Whitley, J C

RESUMO

The genome of Mycoplasma mycoides subsp. mycoides GC1176-2 was cleaved into six large fragments by the endonuclease KpnI which also cleaved the transposon Tn916 once. This has allowed genomic mapping of insertion sites for 50 transformants of GC1176-2 containing Tn916. Almost all of the mapped sites were clearly separate. The transformants provide a bank of genomes each with a KpnI site at a different position to facilitate mapping of gene loci.

Documentos Relacionados

• Conjugal transfer of transposon Tn916 from Streptococcus faecalis to Mycoplasma hominis.
• Transposon Tn916 mutagenesis in Clostridium botulinum.
• Transposon Tn916 mutagenesis in Bacillus anthracis.
• Excision of the Conjugative Transposon Tn916 in Lactococcus lactis
• Interactions of the Integrase Protein of the Conjugative Transposon Tn916 with Its Specific DNA Binding Sites