Ligase-Defective Bacteriophage T4 I. Effects on Mutation Rates
AUTOR(ES)
Koch, Robert E.
RESUMO
Temperature-sensitive mutations in bacteriophage T4 gene 30 (polynucleotide ligase) were examined for their effects on spontaneous and proflavine-induced frameshift mutagenesis in the rII and ac (acridine resistance) cistrons. Only small (fourfold or less) effects on mutation rates were observed, even when selection artifacts involving suppression of gene 30 mutations by rII mutations were taken into account. The deoxyribonucleic acid ligase gene of T4 therefore appears to be only a minor determinant of frameshift mutation rates. This result is consistent with the particular nature of frameshift mutagenesis in bacteriophage T4.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=355057Documentos Relacionados
- Ligase-Defective Bacteriophage T4 II. Physiological Studies
- Mechanism of DNA chain growth, II. Accumulation of newly synthesized short chains in E. coli infected with ligase-defective T4 phages.
- MECHANISM OF DNA CHAIN GROWTH, II. ACCUMULATION OF NEWLY SYNTHESIZED SHORT CHAINS IN Escherichia coli INFECTED WITH LIGASE-DEFECTIVE T4 PHAGES
- cdc9 ligase-defective mutants of Saccharomyces cerevisiae exhibit lowered resistance to lethal effects of bleomycin.
- Nature of φX174 Linear DNA from a DNA Ligase-Defective Host