LC AND UV DETERMINATION OF ALCALOID AND FLAVONOID Passiflora alata CURTIS, Passifloraceae EXTRACTS / "DETERMINAÇÃO DE ALCALÓIDES E FLAVONÓIDES ATRAVÉS DE CLAE E UV DE EXTRATOS DE Passiflora alata CURTIS, Passifloraceae - MARACUJÁ-DOCE".

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Leaves of Passiflora alata Curtis (official species in the Brazilian Pharmacopoeia) are employed for anxiety, as antispasmodic and sedative. The development of new analytical methodologies for the analysis of flavonoids and alkaloids in Passiflora species are needed in order to improve the quality assurance of the plant and derived extract and phytomedicines. A survey on the flavonoids and alkaloids profile and content of isovitexin in leaves and fluid extract of P. alata through LC was made, comparing its chromatographical profiles with a commercial P. incarnata fluidextract and P. actinia tincture. Additionally, the concentration of the total flavonoids of extracts and leaves according to phamarcopoeial photometrical method was determined. The fluidextract of P. alata was produced in accordance with the Pharmacopoeia Helvetica, tincture of P. actinia as produced in accordance to the Brazilian Pharmacopoeia method. The presence of isovitexin in the species (which have distinct chromatographic profiles) was evidenced, which is the major flavonoid compound in the P. incarnata comercial extract (1.198% w/w), but not in P. alata (0.018 summer e 0.007 winter % w/w) fluidextract and leaves (1.137 e 0.018% w/w) P. actinia (0.028 extract and leaves 0.631% w/w). Just traces of vitexin could be observed in the P. alata and P. incarnata (0.312% w/w) and absence of the other tested flavonoids orientin, swertisin, hyperoside and rutin. The LC developed method demonstrated to be adjusted for the qualitative and quantitative analyses and to differentiate species, appropriate to be applied to commercial sample analysis. The flavonoids spectrophotometrical results of three different methods described to P. incarnata were not comparable, the most suitable performance was verified to the British Pharmacopoeia method. None of the classical beta-carbolinic alkaloids tested were found in the P. alata leaves and medicinal extracts: harmane, harmine, harmol, harmalol, harmaline.

ASSUNTO(S)

clae flavonóides alcalóides hplc controle de qualidade passiflora farmacia passiflora quality control flavonoids alkaloids farmacognosia

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