Laserterapia e farmacoterapia com relaxante muscular em masseter de ratos submetidos a desgaste oclusal: análise comparativa através do met e espectroscopia de raman.

AUTOR(ES)

Marcio Vieira Lisboa

DATA DE PUBLICAÇÃO

2008

RESUMO

The role of occlusion on Tempormandibular Disorders (TMD) is still unclear, mainly regarding muscular function, therefore non invasive and reversible treatment, as lasertherapy, may be a valuable instrument in this function. The aim of this study was to evaluate the lasertherapy action on masseter muscle submitted to occlusal alteration. Forty Wistar rats were randomly divided in four groups: 10 for control group, 10 for occlusal alteration group (CCO), 10 for CCO + lasertherapy group (l830nm, 4J/cm2) and 10 for CCO + muscle relaxant group (2,5 mg/Kg Dantrolene). Rats underwent unilateral amputation of the left inferior and superior molar cusps to simulate an occlusal wear situation. The rats of control group had no occlusal wear. Half of the animals of each group was sacrificed in 14 days after the occlusal consuming and half 30 days after the occlusal consuming. The masseter muscles ipsilateral to the amputated molars were excised and processed for light microscopy, electron microscopy, and evaluation of calcium phosphate precipitate by Ramam spectroscopy. The light microscopy did not show differences between the groups. The electron microscopy was able to detect a degree of intracellular damage in muscle fibers of CCO group: swollen mitochondria with disrupted cristae and cleared matrix, signs of hypercontraction of I bands and myofibril disorganization. A decrease in Raman intensity for calcium phosphate precipitate was detected in 30 days laser group. Based on the results, there were alterations in muscular fibers of ipsilateral masseter with occlusal unilateral wear in Wistar rats, although the lasertherapy and pharmacotherapy played few action on treatment of masseter muscle in the studied conditions.

ASSUNTO(S)

raman spectrum analysis oclusão dentária músculo masséter dental occlusion análise espectral raman odontologia laser masseter muscle laser

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