Large T antigens of simian virus 40 and polyomavirus efficiently establish primary fibroblasts.

AUTOR(ES)

Jat, P S

RESUMO

Recombinant retroviruses that transduce the simian virus 40 (SV40) large T antigen or the polyomavirus large T antigen as well as encoding resistance to antibiotic G418 were used to investigate whether these genes alone were sufficient for immortalization of primary cells. The results provided definitive evidence that either viral gene can efficiently establish primary fibroblasts. The capability of the SV40 large T antigen to establish primary fibroblasts was undiminished by a mutation that alters its binding to sequences within the origin of replication. Surprisingly, most of the primary cells established by the expression of the SV40 large T antigen did not have a transformed phenotype. This suggests that transformation by SV40 is not simply due to a high level of expression of the SV40 large T antigen and stabilization of cellular p53.

Documentos Relacionados

• Sequences from polyomavirus and simian virus 40 large T genes capable of immortalizing primary rat embryo fibroblasts.
• Recombinant retroviruses encoding simian virus 40 large T antigen and polyomavirus large and middle T antigens.
• Polyomavirus and simian virus 40 large T antigens bind to common DNA sequences.
• Murine polyomavirus and simian virus 40 large T antigens produce different structural alterations in viral origin DNA.
• The Simian Virus 40 Small-t and Large-T Antigens Jointly Regulate Cell Cycle Reentry in Human Fibroblasts