Laparoscopic guided local injection in the X-linked muscular dystrophy mouse (mdx) diaphragm. An advance in experimental therapies for Duchenne Muscular Dystrophy

AUTOR(ES)

Lessa, Thais Borges, Carvalho, Rafael Cardoso, Spagnolo, Júlio David, Silva, Luis Claudio Lopes Correia da, Cortopassi, Silvia Renata Gaido, Ambrósio, Carlos Eduardo

FONTE

Acta Cir. Bras.

DATA DE PUBLICAÇÃO

2014-11

RESUMO

PURPOSE:To investigate the development of a laparoscopy technique for local injection into the X-linked muscular dystrophy (mdx) diaphragm.METHODS:It was used 10 mice Balb/C57 and 5 mdx mice and three differents decubitus type were tested: the right lateral, supine, and supine decubitus with 20 degrees elevation of the forelimb. Abdominal caudal face and the 10 intercostal space were tested as spot to introduce the needle into the diaphragm.RESULTS: Supine position with elevation of 20 degrees forelimb and the 10th intercostal space are the beneficial position to apply a local injection.CONCLUSION:It was proved to be possible to perform the laparoscopy technique in the X-linked muscular dystrophy diaphragm and this requires a specific position and technique during the surgery.

Documentos Relacionados

• X chromosome-linked muscular dystrophy (mdx) in the mouse.
• Unusual type of benign x-linked muscular dystrophy.
• The detection of carriers of benign (Becker-type) X-linked muscular dystrophy.
• A mouse model for X-linked adrenoleukodystrophy
• Identification of an X-linked member of the Odc gene family in the mouse.