Lack of detection of human papillomavirus DNA in male urine samples.
AUTOR(ES)
Geddy, P M
RESUMO
OBJECTIVES--To evaluate polymerase chain reaction (PCR) methodology for the detection of urethral human papillomavirus (HPV) infection by examining urinary sediment from males. SETTING--Department of Genitourinary Medicine, Leeds General Infirmary. SUBJECTS--73 male patients attending for treatment of sexually transmitted diseases, including 14 patients with genital warts which did not involve the urethral meatus. METHODS--Urinary sediment was tested for HPV DNA and human beta globin gene DNA by PCR methodology. A consensus primer set capable of detecting a wide range of HPV types was used. PCR product was analysed by gel electrophoresis and ethidium bromide staining. RESULTS--HPV DNA was not detected in any of the specimens. Human beta globin gene DNA was identified in 40 of the 73 specimens (55%). CONCLUSIONS--Screening urinary sediment for HPV DNA by PCR methodology with analysis of PCR product by gel electrophoresis and ethidium bromide staining is probably unhelpful for studying the prevalence of urethral HPV infection in men.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1195087Documentos Relacionados
- Human papillomavirus DNA in urine samples compared with that in simultaneously collected urethra and cervix samples.
- Human papillomavirus detection in urine samples from male patients by the polymerase chain reaction.
- Detection of Chlamydia trachomatis in general practice urine samples.
- Evaluation of an enzyme-linked immunoassay and confirmatory test for the detection of Chlamydia trachomatis in male urine samples.
- Dot immunoperoxidase assay using monoclonal antibody for direct detection of cytomegalovirus in urine samples.