Karyotyping of Cryptococcus neoformans as an epidemiological tool.
AUTOR(ES)
Perfect, J R
RESUMO
Karyotyping of Cryptococcus neoformans var. neoformans can be used as an epidemiological tool for C. neoformans infections. In this study of over 40 isolates from both clinical and environmental sources, 90% had a unique chromosome banding by pulsed-field electrophoresis. There was no conserved pattern associated with body site of infection, geographical location of the isolate, or human immunodeficiency virus status. Karyotypes of individual isolates remained stable during both in vitro passage and in vivo infections. Karyotype was used to exclude the possibility of nosocomial spread of C. neoformans in one clinical situation and supported relapse in two other cases. Because of its variable sizes between isolates, karyotyping of C. neoformans is a convenient method for molecular identification of different strains.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=266409Documentos Relacionados
- Acrylamide gel electrophoresis of proteins of Neisseria gonorrhoeae as an epidemiological tool.
- Pyocin sensitivity of Neisseria gonorrhoeae and its feasibility as an epidemiological tool.
- Pseudomonas aeruginosa enzyme profiling: predictor of potential invasiveness and use as an epidemiological tool.
- The medical audit as an operational tool.
- Molecular cloning of a highly repeated DNA element from Mycobacterium tuberculosis and its use as an epidemiological tool.