Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
AUTOR(ES)
Thongsahuan, Sorawat, Baimai, Visut, Otsuka, Yasushi, Saeung, Atiporn, Tuetun, Benjawan, Jariyapan, Narissara, Suwannamit, Sittiporn, Somboon, Pradya, Jitpakdi, Atchariya, Takaoka, Hiroyuki, Choochote, Wej
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2009-07
RESUMO
Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2 generations, suggesting a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies variation (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms.
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