Isotype specific immune responses in murine experimental toxocariasis
AUTOR(ES)
Cuéllar, C, Fenoy, S, Águila, C del, Guillén, JL
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2001-05
RESUMO
In this work, a murine experimental model of toxocariasis has been developed in BALB/c, C57BL/10 and C3H murine strains orally inoculated with 4,000 Toxocara canis embryonated eggs, in order to investigate the isotype-specific immune responses against excretory-secretory antigens from larvae. T. canis specific IgG+M, IgM, IgG, IgA, IgG1, IgG2a and IgG3 were tested by ELISA. The dynamics of the specific immunoglobulins (IgG+IgM) production showed a contrasting profile regarding the murine strain. Conversely to the results obtained with the IgM isotype, the IgG antibody class showed similar patterns to those obtained with IgG+IgM antibodies, only in the case of the BALB/c strain, being different and much higher than the obtained with IgG+IgM antibodies, when the C3H murine strain was used. The antibodies IgG+IgM tested in BALB/c and C57BL/10 were both of the IgM and IgG isotypes. Conversely, in the C3H strain only IgG specific antibody levels were detected. The IgG1 subclass responses showed a similar profile in the three murine strains studied, with high values in BALB/c, as in the case of the IgG responses.
Documentos Relacionados
- Experimental Murine Candidiasis: Pathological and Immune Responses in T-Lymphocyte-Depleted Mice
- Interleukin 2 enhances specific and nonspecific immune responses in experimental Chagas' disease.
- 4-1BB (CD137) Differentially Regulates Murine In Vivo Protein- and Polysaccharide-Specific Immunoglobulin Isotype Responses to Streptococcus pneumoniae
- Immune responses in experimental allergic neuritis.
- Experimental murine candidiasis: pathological and immune responses to cutaneous inoculation with Candida albicans.