Isolation of the human insulin-like growth factor genes: insulin-like growth factor II and insulin genes are contiguous.
AUTOR(ES)
Bell, G I
RESUMO
Overlapping recombinant clones that encompass the insulin-like growth factor (IGF) I and II genes have been isolated from a human genomic DNA library. Each gene is present once per haploid genome; the IGF-I gene spans greater than 35 kilobase pairs (kbp) and the IGF-II gene is at least 15 kbp. The exon-intron organization of these genes is similar, each having four exons, which is one more than the related insulin gene. Comparison of the restriction endonuclease cleavage maps of the IGF-II and insulin genes, including their flanking regions and hybridization with an IGF-II cDNA probe, revealed that they are adjacent to one another. The IGF-II and insulin genes have the same polarity and are separated by 12.6 kbp of intergenic DNA that includes a dispersed middle repetitive Alu sequence. The order of the genes is 5'-insulin-IGF-II-3'.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=390734Documentos Relacionados
- The receptor for insulin-like growth factor II mediates an insulin-like response.
- Insulin-like growth factor II-mediated proliferation of human neuroblastoma.
- Expression of insulin-like growth factor-I and -II genes in human smooth muscle tumours.
- Insulin-like growth factor II signaling through the insulin-like growth factor II/mannose-6-phosphate receptor promotes exocytosis in insulin-secreting cells
- Insulin-like growth factors and insulin-like growth factor binding proteins in mammary gland function
