Isolation of L-Forms of Bacillus subtilis Which Grow in Liquid Medium
AUTOR(ES)
Young, Frank E.
RESUMO
L-forms of Bacillus subtilis can be isolated by treatment of the parent strain sequentially with N-methyl-N′-nitro-N-nitrosoguanidine and lysozyme and selection of the surviving protoplasts on semisolid medium containing 2,000 units of penicillin per ml. Some of these clones can be adapted to grow in liquid cultures containing 1.2 m NaCl. This method will aid in the isolation of cell wall mutants which require hypertonic medium for growth.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=247639Documentos Relacionados
- Time-Lapse Photography of Bacillus subtilis L-Forms Replicating in Liquid Medium
- Adaptation of Meningococcal L-Forms to Agar-free Liquid Medium1
- Isolation of L-forms by blood culture.
- GROWTH OF L-FORMS OF PROTEUS MIRABILIS IN LIQUID MEDIA
- Design of a Microculture Chamber to Observe Cell Division of Bacterial L-Forms in Liquid Medium