Isolation of intact high-molecular-weight DNA by using guanidine isothiocyanate.
AUTOR(ES)
Lippke, J A
RESUMO
A method for obtaining high-molecular-weight chromosomal DNA from Bacteroides intermedius and Bacteroides gingivalis is described. This technique is a modification of the guanidine isothiocyanate isolation procedure for RNA and should be useful for isolating intact DNA from organisms with high endogenous nuclease activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=204150Documentos Relacionados
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