Isolation of cDNA clones derived from a cellular gene transcriptionally induced by herpes simplex virus.
AUTOR(ES)
Patel, R
RESUMO
A small number of cellular proteins accumulate to high levels in cells infected with Herpes Simplex Virus (HSV) despite a generalised repression of most host cell bio-synthesis. An antibody to one such protein has been used to screen a lambda gt11 library and for polysome immunoprecipitation in order to isolate cDNA clones derived from the corresponding gene. The cDNA clones have been used in dot blot and nuclear run-off assays to show that HSV, like other DNA tumour viruses can transcriptionally induce a cellular gene. The mechanism of this effect which is dependent on viral protein synthesis and its possible significance in transformation by HSV are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=311581Documentos Relacionados
- Isolation and characterization of a functional cDNA encoding ICP0 from herpes simplex virus type 1.
- Isolation of a herpes simplex virus cDNA encoding the DNA repair enzyme uracil-DNA glycosylase.
- The cDNA of UL15, a highly conserved herpes simplex virus 1 gene, effectively replaces the two exons of the wild-type virus.
- Regulation of cellular genes transduced by herpes simplex virus.
- Infectious RNA derived by transcription from cloned cDNA copies of the genomic RNA of an insect virus.