Isolation of Bacteria, Transforming Bacteria, and Bacteroids from Soybean Nodules 1
AUTOR(ES)
Ching, Te May
RESUMO
Postnuclei supernatant of soybean (Glycine max cv. Chippewa 64) nodule homogenate was fractionated by stepwise sucrose density gradient centrifugation into supernatant, endoplasmic reticulum and mitochondria, and three distinct bands with 1.22, 1.25, and 1.27 g/cm3 of peak density. Based on their enzymic activities, composition of electron transport components, and ultrastructural characteristics, the lightest band appears to be the mature bacteroids; the intermediate band the transforming bacteria; and the heaviest, the bacteria. The isolation procedure separates nodule symbionts into different functional and developmental fractions, and it may be a valuable tool for studies involving development, regulation, and senescence of bacteroids in the nodule.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=542712Documentos Relacionados
- Carbohydrate, Organic Acid, and Amino Acid Composition of Bacteroids and Cytosol from Soybean Nodules 1
- Properties of the nitrogenase system in cell-free extracts of bacteroids from soybean root nodules.
- Formation of Novel Polysaccharides by Bradyrhizobium japonicum Bacteroids in Soybean Nodules
- Bacteroids Are Stable during Dark-Induced Senescence of Soybean Root Nodules 12
- Cyclic [beta]-1,6 -1,3 Glucans Are Synthesized by Bradyrhizobium japonicum Bacteroids within Soybean (Glycine max) Root Nodules.