Isolation and quantitative HPLC-PDA analysis of lupeol in phytopharmaceutical intermediate products from Vernonanthura ferruginea (Less.) H. Rob.

AUTOR(ES)

Oliveira, Ezequiane Machado Silva, Freitas, Sabrine Lemes, Martins, Frederico Severino, Couto, Renê Oliveira, Pinto, Mariana Viana, Paula, José Realino, Conceição, Edemilson Cardoso, Bara, Maria Teresa Freitas

FONTE

Química Nova

DATA DE PUBLICAÇÃO

2012

RESUMO

Prior to obtain a standardized dried extract from V. ferruginea, lupeol was first time isolated from leaves and used as chemical maker. An analytical method using HPLC-PDA for lupeol determination in V. ferruginea intermediate products was developed using a C8 reverse-phase column, acetonitrile-acetic acid (99.99:0.01, v/v) as mobile phase at 0.8 mL min-1, oven temperature at 23-25 ºC, sample injection volume at 30 µL and detection at 210 nm. The method presented linearity from 10 to 160 µg mL-1, accuracy, precision, robustness and suitable sensitivity proving to be a useful tool to the obtainment process of lupeol standardized dried extracts of V. ferruginea.

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