ISOLATION AND PARTIAL CHARACTERIZATION OF A MAJOR INOSITOL-CONTAINING LIPID IN BAKER'S YEAST, MANNOSYL-DIINOSITOL, DIPHOSPHORYL-CERAMIDE*

AUTOR(ES)

Steiner, S.

RESUMO

A polar lipid containing about 20 per cent of the lipid soluble inositol has been purified from cells of Saccharomyces cerevisiae. This compound labeled with 3H-inositol, 32Pi, or 14C glucose was isolated from aqueous pyridine extracts by means of paper chromatography. Unlabeled compound was obtained by a differential solubility scheme. Analytical and degradation studies suggest that this compound can be formulated as a mannosyl-diinositol, diphosphoryl-ceramide. It can be converted by treatment with base or acid into a mannosylmonoinositol phosphoryl-ceramide, a composition which relates it to the basic structure of the phytoglycolipids.

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