Isolation and Morphology of Temperate Agrobacterium tumefaciens Bacteriophage1

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Zimmerer, Robert P. (The Pennsylvania State University, University Park), Robert H. Hamilton, and Christine Pootjes. Isolation and morphology of temperate Agrobacterium tumefaciens bacteriophage. J. Bacteriol. 92:746–750. 1966.—Lysogeny was detected in 14 strains of Agrobacterium tumefaciens among 130 bacterial strains tested with strain B-6 used as the host. Partial lysis was observed with 13 additional bacterial strains. Morphological studies of five strains showed that the phage had similar features. A typical phage (Lv-1) had a polyhedralshaped head, approximately 71 by 63 mμ, and a tail, approximately 211 mμ by 9.5 mμ. The phage nucleic acid was found to be deoxyribonucleic acid. The bacteriophage have been designated L (for lysogenic) followed by the bacterial strain designation in lower case letters.

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