Isolation and Identification of Canthaxanthin from Micrococcus roseus

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Cooney, J. J. (University of Dayton, Dayton, Ohio), H. W. Marks, Jr., and Anne M. Smith. Isolation and identification of canthaxanthin from Micrococcus roseus. J. Bacteriol. 92:342–345. 1966.—The principal colored carotenoid of Micrococcus roseus was purified by solvent partitioning followed by column and thin-layer chromatography. Absorption spectra, partition coefficients, and infrared spectra suggested that the pigment was a diketo derivative of β-carotene. The pigment was subjected to reduction, and the reduced pigment was subsequently dehydrated. Spectral data and partition coefficients of these derivatives indicated that the original pigment was canthaxanthin (4′,4′-diketo-β-carotene). The pigment was an all-trans isomer; it does not exist as an ester in M. roseus. Canthaxanthin has not previously been identified as a bacterial pigment.

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