Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil

AUTOR(ES)

Silva Filho, Osmar Ferreira da, Argôlo Neto, Napoleão Martins, Carvalho, Maria Acelina Martins de, Carvalho, Yulla Klinger de, Diniz, Anaemilia das Neves, Moura, Laécio da Silva, Ambrósio, Carlos Eduardo, Monteiro, Janaína Munuera, Almeida, Hatawa Melo de, Miglino, Maria Angélica, Alves, Jacyara de Jesus Rosa Pereira, Macedo, Kássio Vieira, Rocha, Andressa Rego da, Feitosa, Matheus Levi Tajra, Alves, Flávio Ribeiro

FONTE

Acta Cir. Bras.

DATA DE PUBLICAÇÃO

2014-08

RESUMO

PURPOSE:To characterize bone marrow progenitors cells grown in vitro,using native goats from northeastern Brazil as animal model.METHODS:Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog).RESULTS:Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog.CONCLUSIONS:Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.

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