Isolation and characterization of cDNA clones encoding a functional p34cdc2 homologue from Zea mays.
AUTOR(ES)
Colasanti, J
RESUMO
We describe the isolation of cDNA clones encoding a p34cdc2 homologue from a higher plant, Zea mays (maize). A full-length cDNA clone, cdc2ZmA, was isolated, sequenced, and shown to complement a cdc28 mutation in Saccharomyces cerevisiae. Comparison of the deduced amino acid sequence of the maize p34cdc2 protein with other homologues showed that it was 64% identical to human p34cdc2 and 63% identical to Schizosaccharomyces pombe and S. cerevisiae p34cdc2 proteins. Studies of expression of the maize cdc2 gene(s) by Northern blot analysis indicated a correlation between the abundance of cdc2 mRNA and the proliferative state of the tissue. Southern blot analysis, as well as isolation of another cDNA clone, cdc2ZmB, which is 96% identical to cdc2ZmA, indicates that maize has multiple cdc2 genes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=51450Documentos Relacionados
- A cDNA clone encoding the 27-kilodalton subunits of glutathione S-transferase IV from Zea mays.
- Nucleotide sequence of a cDNA encoding the beta subunit of the mitochondrial ATP synthase from Zea mays.
- Isolation of a full-length cDNA encoding Zea mays gamma-tubulin.
- cDNA clones of the auxin-binding protein from corn coleoptiles (Zea mays L.): isolation and characterization by immunological methods.
- Isolation and sequence analysis of a cDNA encoding the ATP/ADP translocator of Zea mays L.
