Isolation and characterization of a microorganism involved on autotrophic denitrification by sulfide oxidation in a vertical fixed-bed reactor / Isolamento e caracterização de microrganismo envolvido na desnitrificação autrófica pela oxidação de sulfeto em reator vertical de leito fixo

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

The aim of this study was to evaluate the community involved on autotrophic denitrification by sulfide oxidation applied to the post-treatment of anaerobic effluents. The enrichment of the bacterial community and autotrophic denitrifier community was accessed in three immobilized bed reactors operated at the conditions of autotrophic reduction of nitrate, autotrophic reduction of nitrite and autotrophic reduction of nitrate under excess of sulfide. Following the determination of the best enrichment conditions, the culture was purified, identified by molecular biology tools and the best growth conditions were characterized. Enriched cultures were obtained for the three operational conditions, but the best condition for the growth of autotrophic denitrifiers microorganisms was at N/Sratio of 0,8. The isolated microorganism was identified as Pseudomonas stutzeri. The maximum specific growth rate (mümáx) was 0.037/h, with a doubling time of 18.7 hours. The growth yield (Y) of nitrogen compound was 0.15 gSSV/gNand the specific rate of nitrogen utilization was approximately 0.24 gN/gSSV.h. The results indicated the viability of application of this microorganism for autotrophic denitrification using sulfide as electron donor.

ASSUNTO(S)

bactérias oxidadoras de sulfeto mpn nmp pcr/dgge pcr/dgge pseudomonas stutzeri pseudomonas stutzeri rrna 16s gene sequencing seqüenciamento do gene rrna 16s sulfide oxidizing microorganisms

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