Isolamento e detecção molecular de riquétsias do Grupo Febre Maculosa, a partir de Amblyomma cajennense (Fabricius, 1787) e espécimens biológicos humanos, provenientes de áreas endêmicas do Estado de São Paulo / SPOTTED FEVER GROUP RICKETTSIAE ISOLATION AND MOLECULAR DETECTION, FROM Amblyomma cajennense (FABRICIUS, 1787) AND HUMAN, FROM ENDEMIC AREAS OF STATE OF SÃO PAULO.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

Brazilian spotted fever (BSF), a disease caused by spotted fever group rickettsiae (SFGR) transmitted by Amblyomma cajennense, is generally fatal when not early treated. Common laboratory methods (Vero cell culture and immunofluorescence indirect assay) are not efficient on the diagnosis in most of the cases observed in State of São Paulo, where the agent is not well characterized yet. By means of PCR and Southern Blotting rickettsial genes (gltA, ompB and ompA) were searched in samples of coagulum sanguineous from 20 presenting symptom patients (São Paulo), from these, 19 of them have presented negative results by conventional methods. About 95% of the patients were positive and the ompB base pair sequences amplified showed 98% of similarity with Rickettsia ricketsii. From the 75 lots of A. cajennense (n=1483), collected at the same endemic region, 9 were positive in Vero cell cultures and in 22 it was possible to detect the ompA gene (SFGR). Sequencing and analysis of the amplified samples can contribute to the complete understanding of the rickettsiae biological cycle.

ASSUNTO(S)

amblyomma cajennense pcr amblyomma cajennense shell vial spotted fever ifi ifi rickettsia rickettsii rickettsia rickettsii febre maculosa shell vial pcr

ACESSO AO ARTIGO

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