Isolamento e caracterização de uma nova espécie de bactéria redutora de sulfato obtida de drenagem ácida de mina / Isolamento e caracterização de uma nova espécie de bactéria redutora de sulfato obtida de drenagem ácida de mina

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Acid mine drainage (AMD) constitute a worldwide environmental problem. AMD waters are highly acidic (pH <4). It also contains high concentrations of sulfate and soluble heavy metals which are very toxic to many living organisms. The use of sulfatereducing bacteria (SRB) is a cost-effective route to treat AMD. The aim objective of this work was to verify the potential isolation SRB from AMD. Firstly, the sediments of AMD and Postgate C liquid medium was used to obtain the enrichment culture C and then use it to isolate one culture which labeled as C.1. This culture was able to grow in 5,5 and 7,0 pH and carried out one expressive reducing sulfate in this conditions. The cloning and sequencing of the genes of the enzymes sulfite reductase and hidrogenase obtained genomic DNA extracted from culture C.1. These sequence were used to the identification of the genus this culture. BLASTn and BLASTp analyses showed 93-95% identity with the species of Desulfovibrio genera. Morphologic, physiologic and phylogenetic analyses suggest that the C.1 strain is a new Desulfovibrio sp. with unexpected sulfate reducing capacity at moderately acidic pH. Also, these results shown new possibilities for the AMD treatment using this strain without a previous neutralization step.

ASSUNTO(S)

recursos hidricos 1. drenagem ácida - teses. 2. minas - drenagem - teses. 3. sulfatos - teses. i. universidade federal de ouro preto. ii. título.

ACESSO AO ARTIGO

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