Isolamento e caracterização de peptídeos antimicrobianos derivados da digestão da hemoglobina em Rhipicephalus (Boophilus microplus) / Isolation and characterization of antimicrobial peptides produced during hemoglobin digestion on Rhipicephalus (Boophilus) microplus.
AUTOR(ES)
Rodrigo Caetano Belmonte da Silva
DATA DE PUBLICAÇÃO
2010
RESUMO
The hemoglobin posses a great potential as an antimicrobial, being denominated hemocidins the antimicrobial peptides (AMPs) derived from heme containing proteins. The cattle tick Rhipicephalus (Boophilus) microplus is capable of processing large amounts of blood to its own metabolism. In this work we purified the hemocidins that are produced in the tick gut. We identified 10 fragments from bovine hemoglobin that presents activity towards Candida albicans (Hb 1-94, 3-94, 1-87, 93-141, 102-141, 103-141, 107-141, 104-141 e 98-114 e Hb"beta" 127-145). We determined that all the peptides presents a high content of basic amino acids, besides a secondary structure in "alfa"-helix conformation, characteristics found in other AMPs. We also verified that a cistein- and an aspartic-protease are the two main proteases involved on the production of these hemocids. The identification of several hemocidins in the gut of R. (Boophilus) microplus, suggests that these AMPs may play a role in the defense of the tick against microorganisms
ASSUNTO(S)
hemoglobin digestion peptídeos antimicrobianos digestão da hemoglobina antimicrobial peptides rhipicephalus (boophilus) microplus sistema imune hemocidinas hemocidins immune system rhipicephalus (boophilus) microplus
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