Isolamento e analise genetica de mutantes de Aspergillus niger com aumento na produção de amiloglicosidase

AUTOR(ES)
DATA DE PUBLICAÇÃO

1988

RESUMO

The aim of this work was the isolation and genetical analysis of mutants with increased amyloglucosidase production using the pab1fwn1 strain of Aspergillus Niger. The mutagenic used was ultraviolet light that showed efficiency in the induction of this type of mutant, which could be isolated after some modifications in the methodology used by VALENT (1985). Four mutants were selected with an increase of about 30% in production, being this difference significant in several tests realized. Tests using AG specific inhibitor revealed that the observed increase in production can be atributed mainly to amyloglucosidase. The chosen denomination for the mutants was hap which stands for high amyloglucosidase production. The hap mutants isolated - hap112, hap147, hap169 and hap252 were crossed with nic1olv3 strain that shows normal production and compatible markers for diploids isolation. These diploids were assayed for enzyme production, aiming to establish the type of allelic interaction. The results showed that the AG production markers present in three mutants could be recessive (hap112, hap147 and _ap169) and in a other mutant could be semi-dorninant (hap252). In order to map the hap genes, segregants of these diploids were isolated and, after assays to examine enzyme production and auxotrofic markers it can be suggested that the genes hap147 and _ap169 are jn the linkage group 1; the gene hap C112 could not be mapped and the hap 252 characteristic can be atributed to two non aIlelic genes located jn two linkage groups as follows: hapA252 jn the linkage group I and hapR252 in the linkage group lI

ASSUNTO(S)

aspergilos microbiologia genetica microbiana

ACESSO AO ARTIGO

Documentos Relacionados