Involvement of 4-hydroxymandelic acid in the degradation of mandelic acid by Pseudomonas convexa.
AUTOR(ES)
Bhat, S G
RESUMO
A microorganism capable of degrading DL-mandelic acid was isolated from sewage sediment of enrichment culture and was identified as Pseudomonas convexa. It was found to metabolize mandelic acid by a new pathway involving 4-hydroxymandelic acid, 4-hydroxybenzaldehyde, 4-hydroxybenzoic acid, and 3,4-dihydroxybenzoic acid as aromatic intermediates. All the enzymes of the pathway were demonstrated in cell-free extracts. L-Mandelate-4-hydroxylase, a soluble enzyme, requires tetrahydropteridine, nicotinamide adenine dinucleotide phosphate, reduced form, and Fe2+ for its activity. The next enzyme, L-4-hydroxymandelate oxidase (decarboxylating), a particulate enzyme, requires flavine adenine dinucleotide and Mn2+ for its activity. A nicotinamide adenine dinucleotide-dependent, as well as a nicotinamide adenine dinucleotide phosphate-dependent, benzaldehyde dehydrogenase has been resolved and partially purified.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=232901Documentos Relacionados
- NIC, a conjugative nicotine-nicotinate degradative plasmid in Pseudomonas convexa.
- Involvement of the Protocatechuate Pathway in the Metabolism of Mandelic Acid by Aspergillus niger
- Degradation of 4-chlorophenylacetic acid by a Pseudomonas species.
- THE DIAGNOSIS OF PHEOCHROMOCYTOMA BY DETERMINATION OF URINARY 3-METHOXY,4-HYDROXYMANDELIC ACID*†
- Molybdenum Involvement in Aerobic Degradation of 2-Furoic Acid by Pseudomonas putida Fu1