Investigation of clinical utility of ATM and PTEN genes in the differentiated thyroid cancer / Avaliação da utilidade clinica dos genes ATM e PTEN em cancer bem diferenciado da tireoide

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

In order to investigate the clinical utility of two tumor-suppressing genes involved in the control of cell proliferation and survival, we ve studied proteins ATM and PTEN through immunohistochemistry in 272 differentiated thyroid carcinoma diagnosed patients (142 classical papillary thyroid carcinomas type, 72 papillary thyroid carcinomas follicular variant, 17 papillary thyroid carcinomas tall cells variant and 41 follicular carcinomas), 106 tissue samples from patients diagnosed with benign thyroid diseases (55 follicular thyroid adenomas and 51 with goiter), 18 normal thyroid tissue samples extracted from the counter lateral lobe of individuals operated for follicular thyroid adenomas. The quantitative expression of ATM gene was available for Real-Time PCR in 87 papillary carcinomas. In order to analyze the genotypic profile, we ve also studied three ATM polymorphisms (D1853N, S707P e S49C) in 199 patients (164 papillary carcinomas and 35 follicular carcinomas) and 219 control individuals. Patients were accompanied for 53,8±41 months, using a standard protocol. 179 patients were tagged as disease-free and 48 patients had bad outcome (12 deaths). The analysis of multiple logistic regression adjusted for gender and age has showed that the ATM protein expression was more frequent among patients that didn t have metastasis when diagnosed (81%) and that were free of disease (63%) (p=0.016; p=0.0276 respectively). The major incidence of cases who expression PTEN protein, also was observed in patients that didn t have metastasis during follow- up (75%) (p= 0.0437). The heterozygous alleles for D1853N polymorphism was more prevailing among the controls (64%) than in individuals with cancer (36%) (p=0.0364). Nevertheless these results demonstrated that ATM and PTEN protein expression can be useful in identifying patients with aggressiveness and the classification of prognosis in differentiated thyroid carcinoma

ASSUNTO(S)

neoplasm dna damage tireoide - tumores dano ao dna thyroid tumors cancer

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