Investigating he signature of natural selection on exonic splicing regulators of the human genome / Análise dos sinais de seleção natural em reguladores de splicing exônicos do genoma humano

AUTOR(ES)

Rodrigo Fernandes Ramalho

FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

25/06/2012

RESUMO

Splicing is the process by which introns are removed from a mRNA precursor and exons are ligated to form a mature mRNA. During this process several cis and trans factors are involved. Besides the canonical cis factors (e.g., splicing sites, branch point and polypyrimidine tract), Splicing Regulators - short sequences located in exons and introns - have an important role in assisting the spliceossome to correctly recognize exon/intron boundaries. Through alternative splicing, great transcript diversity is generated, and currently it is known that more than 80% of human genes present splicing variants. The evolution of Exonic Splicing Regulators (ESRs) has been mainly analyzed by interspecific comparisons. Although these studies have revealed consistent results (evidences of weak negative selection against synonymous variations that affect ESRs), other findings still appear inconsistent, for instance the reports on increased level of conservation and higher non-synonymous evolutionary rate in alternative than constitutive exons. The present thesis investigates the regime and intensity of natural selection on exons and their ESRs by comparing intra and interspecific genetic variation. We demonstrate that 1. ESRs from constitutive and alternative exons differ significantly in density and the intensity of negative selection. 2. The exonic splicing silencers have a major role in the origin of exons skipping from constitutive exons, and also on events of alternative usage of splicing sites. 3. There is a positive correlation between the exon inclusion level and the intensity of negative selection against non-synonymous variations

ASSUNTO(S)

evolução evolution natural selection reguladores de splicing seleção natural splicing regulators

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