Investigação dos efeitos toxicos e anti-hipertensivo de Arrabidaea chica Verlot (Bignoniaceae)

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

The species Arrabidaea chica (Bignoniaceae) is indicated to treatment of skin illnesses, renal stones, systemic arterial hypertension, as an antiinflammation and antianemia. In this paper it was avaliated the toxical effect of the extract of leaves of Arrabidaea chica Verlot (EAC), the chemical composition of EAC and the performance of EAC n the cardiovascular system of normotense and hypertense rats SHR. To the preparation of the EAC it was used dried pulverized leaves, macerated in ethanol 70%. After that the obstention of the EAC this was fractioned when it obtained several fractions among them the F63, this because of presenting in chromatrography compared and thinned. It was cleaner and in a major quantity. It was analysed in farmacological experiments, 1RMNH and CLAE. In the chemical composition of the EAC, it was detected the presence of flobafenical tanines, flavonos and steroidal componnds. In the the fraction F63 it was observed characteristic signals as to the alifatical componnds and aromatical. Among several possibilities they can beflavonoidal. To the avaliation of the acute toxicity (DL50) adult mice of both sexes received saline (0,1ml/10 g v. o) or EAC (0,5m 1,0 e 2,0 g/kg, ip), this didnt cause the death of animals as well it didnt get the behaviour alterations or macroscopic alterations of organs if compored to the control group. The treatment of rats with saline (0,1 mL/ 100g) and EAC (0,5,-5,0 g/kg, v.o), didnt cause deaths, nor behaviour or macroscopic alterations of the organs after antopsy. Finished the period of 14 days of treatment the blood was collected to its hematological and biochemical analysis, there wasnt difference between the control groups and treated in relation to the ration consume, corporal weight as well it wasnt detected alterations of behaviour or in the macroscopic analysis of the organs. The EAC (1,0 g/ kg) promoted meanful alteration in the VCM and in the doses of hemoglobine. The EAC of Arrabidaea chica Verlot (1 g/kg) promoted meanful alterations in the creatine, urea and cholesterol (HDL), in the dose of 0,5 g/Kg. It promoted, also, meanful alterations in the urea and in the HDL. The EAC (2,0 g/ kg) altered the glicosis, AST and total cholesterol, as to the dose of 3,5 g/Kg the EAC altered glicosis and total cholesterol. The EAC (5,0 g/kg) promoted alterations of glicosis, AST, urea and HDL the EAC (0,1; 0,2 e 0,5 g/kg, v.o.) administrated to hypertense rats SHR produced hypotension (38,2%; 49,9% and 34,4%) and bradicardia (13,4%; 15,35 % and 15,25%) as to the controls. In normotense animals there was reduction of the tension pressorical levls without alterations of the cardial frequence. In the menseterical superior arteries isolated of normotense rts or hypertense SHR submitted to chronical treatment with EAC for 60 days it was possible to detect reductions of the maximal effect and displacement of the curve of presence of noradrenalin when compared to the tissues of the respective control groups. In the analysis in vitro performed in rings of mesenterical superior artery isolated of rats with and without functional endothelial, in the presence and absence of the EAC (100-500 μg/mL) and F63 (10-30μg/mL) ocorred reduction of the maximal effect in the three used doses, in a way that the dependent dose and statistically meanful action in preparations of the mensenteric pre-contractd artery with moradrenalin, the EAC (10-500 μg/mL) and F63 (10-50μg/mL) promoted the dependent relaxing of dose both in the presence of the endotelial and in the absence of it. In the presence of L-NAME or indometacine, the EAC didnt promoted difference of the vasorelaxing. The EAC promoted a relaxing in precontracted preparations with KCI 80 mM bolh in the presence and in the absence vascular endotelial. The EAC (50-500 μg/mL) and F63 (10-30 μg/mL) reducted the maximal induced contractions per CaCl2. In precontracted preparations with S-(-)BayK 8644, the EAC (10-500 μg/mL) and F63 (10-300 μg/mL) promoted relaxing. The induced contractions per caffeine in a free ambience of calcium were inhibited by EAC, but the F63 didnt promoted alterations of the induced contractions by noradreline or caffeine. In a set these results suggest that the hypotension produced by the EAC of Arrabidaea chica Verlot can be realted to a diminution of the periferical total resistence in consequence of the participation of the bhlocade of the canaes CaV1.2 in cells of the vascular smooth muscle as well as the blocade of the rianodine channels.

ASSUNTO(S)

farmacologia mesenterical artery arrabidaea chica verlot arrabidaea chica verlot artéria mesentérica hipertensão (shr) hipertension (shr)

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