Polymorphisms in the TGFB1, CD14, IL4, IL4-Ra, e ADAM33 genes and asthma severity / Investigação da associação entre os polimorfismos dos genes : TGFB1, CD14, IL4, IL4-Ra, e ADAM33 com a gravidade da asma atopica entre crianças e adolescentes

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Asthma is a chronic inflammatory disease affecting air pathways, in which several cells, such as eosinophils, mastocytes and T lymphocytes, play fundamental roles. The inflammatory process is associated with bronchial hyperresponsiveness to a variety of stimulus types. Asthma is a complex and multifactorial genetic disease that does not follow the monogenic line of hereditary patterns. Linkage studies suggest that multiple genes are involved in its pathogenesis. The objective of this work was to verify, in a sample of patients with mild, moderate and acute asthma, a possible association between TGF- beta 1, CD14, IL-4, IL-4Ra, ADAM33 gene polymorphisms and asthma severity. The T869C polymorphism of the TGF- beta 1 gene was analyzed using the ARMS-PCR technique. The other polymorphisms C-509T of the TGF- beta 1 gene, C-159T of the CD14 gene, C-590T of the IL-4 gene, Ile50Val of the IL-4Ra gene , S2 of the ADAM33 gene were be detected by PCR + restriction analysis. The T869C polymorphism (TGF- beta 1) in our sample showed an association between the CC genotype and the patients who presented acute asthma. No association was found concerning the C-509T (TGF- beta 1) polymorphism. Comparing the genotype distribution of the C-159T (CD14) polymorphism on the control group of acute asthma, a significant result with TT and CT genotypes was observed. There was no association between C-590T (IL4) and asthma. There was a significant association between Val/Val (IL-4Ra) genotype and mild asthma. The GC heterozygote of the S_2 polymorphism (ADAM33) with acute and mild asthma. The CC homozygote and mild asthma. The results indicated that T869C (TGF-?1) and C-159T (CD14) polymorphisms can modulate asthma severity

ASSUNTO(S)

asma gene genes polymorphisms asthma polimorfismos (genetica)

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