Intragenic Suppressors of Induction-Deficient TetR Mutants: Localization and Potential Mechanism of Action

AUTOR(ES)

Biburger, Markus

FONTE

American Society for Microbiology

RESUMO

Eight Tn10 Tet repressor mutants with an induction-deficient phenotype and with primary mutations located at or close to the dimer interface were mutagenized and screened for inducibility in the presence of tetracycline. The second-site suppressors with wild-type-like operator binding activity that were obtained act, except for one, at a distance, suggesting that they contribute to conformational changes in the Tet repressor. Many of these long-range suppressors occur along the dimer interface, indicating that interactions between the monomers play an important role in Tet repressor induction.

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