Interferon inhibits hepatitis B virus replication in a stable expression system of transfected viral DNA.
AUTOR(ES)
Hayashi, Y
RESUMO
The effect of interferon (IFN) on hepatitis B virus (HBV) replication was investigated in a stable expression system, using HepG2 cells transfected with recombinant HBV DNA. IFN was found to cause a marked reduction in the levels of both minus and plus strands of HBV DNA from core particles in the cytoplasm. Neither HBV DNA from virus particles nor the HBV surface antigen in the culture medium primarily underwent change in quantity by treatment with IFN, as was also found for HBV mRNAs and the HBV core antigen/HBV e antigen in the cytoplasm. IFN exerted no influence on HBV DNA synthesis by endogenous DNA polymerase in the core particle fraction. From these findings, it would appear that IFN inhibits HBV replication by blocking some step in the pregenome RNA-primed assembly of core particles.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=250847Documentos Relacionados
- Stable expression and replication of hepatitis B virus genome in an integrated state in a human hepatoma cell line transfected with the cloned viral DNA.
- Hepatitis B virus (HBV) particles are produced in a cell culture system by transient expression of transfected HBV DNA.
- Continuous expression and replication of the hepatitis delta virus genome in Hep G2 hepatoblastoma cells transfected with cloned viral DNA.
- Production of hepatitis B virus particles in Hep G2 cells transfected with cloned hepatitis B virus DNA.
- Expression of hepatitis B surface antigen in unselected cell culture transfected with recircularized HBV DNA.