Interaction of Shigella flexneri IpaC with Model Membranes Correlates with Effects on Cultured Cells

AUTOR(ES)

Tran, Ngoc

FONTE

American Society for Microbiology

RESUMO

Invasion of enterocytes by Shigella flexneri requires the properly timed release of IpaB and IpaC at the host-pathogen interface; however, only IpaC has been found to possess quantifiable activities in vitro. We demonstrate here that when added to cultured cells, purified IpaC elicits cytoskeletal changes similar to those that occur during Shigella invasion. This IpaC effect may correlate with its ability to interact with model membranes at physiological pH and to promote entry by an ipaC mutant of S. flexneri.

Documentos Relacionados

• Structural Characterization of the N Terminus of IpaC from Shigella flexneri
• Functional analysis of the Shigella flexneri IpaC invasin by insertional mutagenesis.
• Homologs of the Shigella IpaB and IpaC invasins are required for Salmonella typhimurium entry into cultured epithelial cells.
• Use of Shigella flexneri ipaC and ipaH Gene Sequences for the General Identification of Shigella spp. and Enteroinvasive Escherichia coli
• Use of Shigella flexneri ipaC and ipaH gene sequences for the general identification of Shigella spp. and enteroinvasive Escherichia coli.