Interação entre fungos toxigênicos (Aspergillus flavus e Fusarium verticillioides) e carunchos (Sitophilus zeamais) em amostras de grãos de milho. / Interaction between toxigenic fungus (Aspergillus flavus e Fusarium verticilliodes) and weevils (Sitophilus zeamais) in samples of maize grains.

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

03/08/2011

RESUMO

The weevils Sitophilus zeamais ability was examined to propagate spores of Aspergillus flavus and Fusarium verticillioides and the production of mycotoxins. Corn grains was conserved in flasks connected by a rubber to form a closed system (side A and B) and divided in six groups: G1 (corn + weevil - side A); G2 (corn + A. flavus - side A); G3 (corn + A. flavus + weevil - side A); G4 (corn + F. verticillioides - side A), G5 (corn + F. verticillioides + weevil - side A) e G6 (corn + A. flavus + F. verticillioides + weevil - side A). The side B contained sterile grains. After 10, 20 and 30 days of incubation were realized: weighing, activity water, mycoflora, determination of mycotoxins, nutritional analysis, scanning electron microscope and Real time PCR-RT. In front of the results was observed the importance of Sitophilus zeamais like a fungus vector and the importance of Good Manufacturing Practices and Stores of grains, to reduce the risks of contamination and deterioration.

ASSUNTO(S)

aflotoxinas corn fungi fungos fungos toxicogênicos microtoxinas milho mycotoxins toxigenic fungi aflatoxin

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