Inseminação artificial intra-uterina transcervical assistida por endoscopia em cadelas da raça Retriever do Labrador

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The aims of the present study were to describe endoscopic transcervical intrauterine artificial insemination (AI) technique in bitches, investigate its viability and difficulties; evaluate pregnancy rate and litter size; and evaluate behavioral and reproductive parameters, as vaginal cytology and serum progesterone assays, to determine time of insemination. Ten Labrador Retriever bitches were submitted to three endoscopic transcervical intrauterine AIs (with 48h intervals), using fresh canine semen. Oestrus and ovulation period were established by behavior evaluations, progesterone assays and vaginal cytology, enabling AIs in optimal timing during oestrus. During AI technique execution, the following aspects were evaluated: cervical os catheterization difficulty, semen deposition resistance, occurrence of semen backflow, and time required to perform the AI. It was concluded that endoscopic transcervical intrauterine AI technique was viable in Labrador Retriever bitches. The rigid cystourethroscope and sheath used fit to perform this technique. The methods employed to evaluate behavioral and reproductive parameters during the estrous cycle were effective to determine time of AI. Serial serum progesterone assays enabled determination of LH surge day, and thus ovulation. Pregnancy rate was 90 % (9 in 10 bitches). Mean litter size was 5,0 ± 2,6 puppies. These results were satisfactory in comparison with previous studies using transcervical intrauterine AI.

ASSUNTO(S)

inseminação artificial teses endoscopia teses sêmen teses cadela reprodução teses

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