InovaÃÃo tecnolÃgica baseada na produÃÃo de biopolÃmero com viabilidade para inoculante rizobiano

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

The biopolymers are polysaccharide from microbial origin, synthesized by bacteria, fungi and yeast. They are also known as gums due to their capacity to produce viscous solutions and gel in an acquoso medium. The production of microbial Exopolysaccharide (EPS) has been an object of intensive research, due to its high application and potential in different fields. However, the polysaccharide synthesized by bacteria from Rhizobium, have been studied to help understand their articipation in the leguminous symbiosis X Rhizobium since these biopolymers seem to determine the symbiotic specificity, however, the precise function of those molecules are still to be seen to. During the process of production and viscosity of polysaccharide, it should be considered from the microorganism being studied to the determination of rotatory parameters, time of incubation, excess or lack of carbohydrates and inocule volume, in which the production medium and its influence on the synthesis is highlighted in the yield and composition of exopolysaccharides. The aim of this research is to evaluate the production and viscosity of biopolymers obtained form 8 isolated Rhizobium sp compared to standard strain Rhizobium tropici (CIAT 899) in different culture timing. The biopolymer was obtained through rotation, in shakers. The conditions were: 28ÂC, 200rpm per 96 and 168 hours in modified YM medium. The following items were evaluated: the production, via dry weight of acquoso solution of biopolymers at 2.5% (v/v) through the technique of dry weight and after drying the product, which was done in a dessicator at 30ÂC, with constant weight. For measuring vi cosity, the samples were identified by using a rotational Brookfield (model RVT, Spindle 1) viscometer at 20ÂC, and shear rate. The results showed that the isolated ones had a bigger production within 168 hours of fermentation time. Besides, by increasing the time, there was also an increase of viscosity for all the isolated ones

ASSUNTO(S)

exopolissacarÃdeos (eps) reologia rizÃbio exopolysaccharide(eps) ciencias biologicas rheology rhizobia

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