Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection

AUTOR(ES)

Coutinho, Leticia, Ferreira, Marcelo Alves, Cosson, Alain, Batista, Marcos Meuser, Batista, Denise da Gama Jaén, Minoprio, Paola, Degrave, Wim M, Berneman, Armand, Soeiro, Maria de Nazaré Correia

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2009-12

RESUMO

Proline racemase is an important enzyme of Trypanosoma cruzi and has been shown to be an effective mitogen for B cells, thus contributing to the parasite's immune evasion and persistence in the human host. Recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro. Here we demonstrate that both anti T. cruzi proline racemase antibodies and the specific proline racemase inhibitor pyrrole-2-carboxylic acid significantly affect parasite infection of Vero cells in vitro. This inhibitor also hampers T. cruzi intracellular differentiation.

Documentos Relacionados

• Host–parasite interactions and the evolution of ploidy
• Roles of metallic ions in host-parasite interactions.
• Periodic and chaotic host-parasite interactions in human malaria.
• Host-parasite interactions with peritoneal macrophages of mice and rats in vitro and in vivo.
• Pathogenesis of Acanthamoeba keratitis: carbohydrate-mediated host-parasite interactions.