Inhibition of Chloroplastic Respiration by Osmotic Dehydration 1
AUTOR(ES)
Willeford, Kenneth O.
RESUMO
The respiratory capacity of isolated spinach (Spinacia oleracea L.) chloroplasts, measured as the rate of 14CO2 evolved from the oxidative pentose phosphate cycle in darkened chloroplasts exogenously supplied with [14C]glucose, was progressively diminished by escalating osmotic dehydration with betaine or sorbitol. Comparing the inhibitions of CO2 evolution generated by osmotic dehydration in chloroplasts given C-1 and C-6 labeled glucose, 54% and 84% respectively, indicates that osmotic dehydration effects to a greater extent the recycling of the oxidative pentose phosphate intermediates, fructose-6P and glyceraldehyde-3P. Respiratory inhibition in the darkened chloroplast could be alleviated by addition of NH4Cl (a stromal alkylating agent), iodoacetamide) an inhibitor of glyceraldehyde-3P dehydrogenase), or glycolate-2P (an inhibitor of phosphofructokinase). It is concluded that the site which primarily mediates respiratory inhibition in the darkened chloroplast occurs at the fructose 1,6-bisphosphatase/phosphofructokinase junction.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1055990Documentos Relacionados
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