Influencia do ultra-som na permeação cutanea da cafeina : estudo em fragmentos de pele e em adipocitos isolados de suinos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

The ultrasound has been extensively used in the last decades for physical therapies and as promoter of cutaneous permeation of drugs. The use of ultrasound as facilitator of cutaneous absorption is knows for phonophoresis or sonophoresis. The aim of present research were to analyze the effect of ultrasound on the cutaneous permeation of caffeine applied locally on the skin, and to analyze the lipolytic response of isolated adipocyte of swine after this treatment. The caffeine inhibits the cAMP-phosphodiesterase which hydrolyzes cAMP, this effect result in an increase of the effect of lipolytic agonists. However, has been also mentioned that the caffeine is able to inhibit the adenosine receptors, inhibiting thus effect antilipolytic, as consequence the use of caffeine leads of the facilitation of the lipolysis. For this study, male swine were used Landrace x Large White with 35 days and approximate weight of 15 kg. After trichotomy of the dorsal area the animals were submitted to the following treatments, for fifteen days: gel; caffeine (5%); ultrasound; ultrasound + caffeine (5%). A 5th area was submitted the intradermic infiltration (mesotherapy) of caffeine (2%). A 6th area served as control and it was not submitted to treatment. At the end of the different treatments the animals were sacrificed and fragments of skin of the different areas were used for histological analysis and 15 g of adipose tissue for adipocytes isolation. The lipolytic activity was analyzed in the isolated adipocytes. The adipocytes were incubated with agonist ?-adrenergic (isoprenaline) and the glycerol production were measured. The mesotherapy was shown effective in the reduction of the thickness of the hypodermis. The ultrasound was shown important accentuator of the cutaneous permeation to the caffeine, evidenced so much by the histological study, where it was observed significant morphologic alterations of the adipose tissue as well as for the increase of the maximal lipolytic response, in adipocytes isolated, without, however to alter the sensibility of the ? adrenergic receptors. Also accomplished analysis of cutaneous permeation of the caffeine in vitro through cells of vertical diffusion using skin of male swine Landrace x Large White with 50 days old and the results obtained also demonstrated an accentuated permeation of the caffeine when associated to the therapeutic ultrasound

ASSUNTO(S)

cafeina ultra-som cosmeticos

ACESSO AO ARTIGO

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