Influencia do tabagismo na secreção de citocinas inflamatorias em pacientes com periodontite cronica severa / Impact of tobaccoism on the cytokine secretion of patients with chronic severe periodontitis

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Tobaccoism is a major risk factor in the development and further progression of periodontitis however the pathogenesis of tobacco-related periodontal disease is not well understood. Thus, the purpose of this study was to evaluate heavy tobacco smoking effects (consuming >20 cigarettes/day) on cytokine secretion by peripheral mononuclear cells (PBMC) obtained from periodontal patients compared with healthy non-smokers donors. PBMC were isolated by Ficoll method from 20mL of blood obtained from 20 subjects distributed in four experimental groups: healthy current smokers (FS), periodontitis current smokers (FP), healthy non-smokers (NFS) and periodontitis non-smokers (NFP) donors. Cells were incubated for 24/48 hours in wells containing 500µL RPMI 1640 and activated with 1,0ng/mL LPS (E.coli). Culture supernatants were assessed for interleukins IL-8, IL-6, IL-10 and tumor necrosis factor TNF- alfa by the ELISA test. Results showed that tobaccoism appears to down-regulate TNF- alfa (p>0,05) secretion by mononuclear cells from FP subjects although did not appear to interfere on IL-8, IL-6 and IL-10 between groups (p>0,05). Within the limits of this study, it could be concluded that heavy tobacco smoking reduces TNF- alfa secretion in patients with chronic severe periodontitis.

ASSUNTO(S)

periodontics periodontia elisa

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