Influencia de polimorfismos em genes de citocinas na morbidade da doença periodontal inflamatoria cronica

AUTOR(ES)
DATA DE PUBLICAÇÃO

2002

RESUMO

Chronic Periodontal Disease (CPD) is characterized by an inflammation in the supporting tissues of the teeth. It is primarily caused by bacteria present on tooth surfaces (biofilm), which adjacent to the gingiva, stimulate host cells to produce immunoinflammatory response. Cytokines represent important pathological mediators, which influence the onset and progression of several chronic inflammatory diseases, including CPD. Polymorphisms in cytokine genes have been associated with enhanced expression of the correspondent cytokines. The aim of this study was to investigate the relationship between polymorphisms in the promoter region of the genes IL2, IL4 and IL 10 and CPD. One hundred and thirteen non related subjects, over 25 years, non smoking individuais, were divided according to the severity levei of periodontal disease: 44 healthy individuais, 31 with moderate and 38 with severe periodontitis. DNA was obtained from epithelial cells through a mouthwash with 5 mL 3% glucose and scraping of oral mucosa. The polymorphisms IL2 (-330), IL4 (-590) and IL10 (-819 e -592) were investigated using PCR-RFLP, and the polymorphism IL10 (-1082) was investigated using DNA sequencing. Polymorphisms in the IL 1 O gene were also analysed as haplotypes. Properly statistical methods were employed to access differences between the studied groups and allelic and genotypic frequencies of each polymorphism. P values <0.05 were considered significant. Significant differences were found in the IL2 (-330) and IL 1 O (-819 and -592) polymorphisms. The analysis of the three polymorphisms in the IL 10 gene arranged as haplotypes revealed significant differences regarding CP. The conclusions of this study were: (a) the polymorphism IL2 (-330) was associated with the severity of CP in the studied population; (b) polymorphisms IL4 (-590) and IL10 (-1082) were not related to CP; (c) polymorphisms IL10 (-819 and -592) were associated with susceptibility to CP; (d) specific haplotypes in the promoter region of IL 10 gene are associated with susceptibility to CP, especially in female subjects. The analysis of the polymorphisms IL2 (-330) and IL4 (-590) permitted an additional genetic study. These polymorphisms were useful as markers in population genetic studies, and could complement data obtained by mitochondrial DNA analysis and analysis of the non-recombining portion of the Y chromosome

ASSUNTO(S)

periodontite inflamação

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