Influência da variabilidade genética sobre biomarcadores de dano oxidativo e não oxidativo em pacientes com doença pulmonar obstrutiva crônica (DPOC)

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

2012

RESUMO

Chronic Obstructive Pulmonary Disease (COPD) is a slowly progressive condition of poor reversibility that is characterized by airflow limitation associated with an abnormal inflammatory response of the lungs. Its development and progression are related to increased oxidative stress, which is an important pathogenic component of airway inflammation in this disease. Thus, this case-control study, aimed to evaluate and quantify DNA (using the alkaline and endonuclease-modified comet assay and micronucleus test in exfoliated oral mucosal ), lipid (TBARS) and protein (DNPH assay) damage, and to evaluate the influence of genetic polymorphisms of genes involved in Phase I metabolism of xenobiotics (CYP1A1 C2453A, CYP2E1 C-1053T), Phase II (GSTM1null, GSTT1null, GSTP1 Ile105Val), and neutralization of ROS (CAT C-262T and GPx1 Pro198Leu) on these biomarkers. The target population comprised 51 individuals with healthy pulmonary function and 51 patients with COPD from the Rio Pardo Valley, RS, Brazil. The patients had higher Damage Index (DI) in the alkaline, Fpg and Endo III-modified comet, (P <0.001, P = 0.023 and P = 0.021, respectively). There was no difference in the formation of MN between the groups (P = 0.651). Patients who had not participated in a pulmonary rehabilitation program showed higher levels of lipid peroxidation than the controls and those who participated in the exercises (P <0.001). Additionally, it was found a trend towards a higher protein carbonylation in these non-adherent patients. All investigated polymorphisms had distinct modulatory effects in the comet assay¿s DI in controls, patients, or both, but not in the micronucleus formation. Aside from highlighting the effects of the COPD¿s typical systemic inflammation, this study stresses the influence of individual genetic variability on non-clastogenic damage in these patients¿ DNA.

ASSUNTO(S)

biomarcadores doença pulmonar obstrutiva crônica variabilidade genética

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