Influence of Silver Nitrate on Somatic Embryogenesis Induction in Arabica Coffee (Coffea arabica L.)
AUTOR(ES)
Rojas-Lorz, Laura
FONTE
Braz. arch. biol. technol.
DATA DE PUBLICAÇÃO
29/08/2019
RESUMO
Abstract The influence of silver nitrate (AgNO3), benzyladenine (BAP), and indole-3-acetic acid (IAA) on low frequency somatic embryogenesis (LFSE) induction in Caturra and Catuaí arabica coffee was evaluated. For the Caturra cultivar, the production of somatic embryos was significantly increased by adding AgNO3 to the semisolid culture medium. The highest average number of somatic embryos for this cultivar was obtained using 6.6 μM BAP, 2.85 μM IAA, and 40 μM AgNO3. In contrast, for the Catuaí cultivar, the highest average number of somatic embryos was obtained using semisolid medium supplemented with 8.8 μM BAP, and 2.85 μM IAA. Using these protocols, somatic embryos were directly induced using leaf sections of in vitro plants of both coffee cultivars within 8 weeks. The somatic embryos developed into rooted plants with a 100% survival rate upon transfer to the greenhouse.
Documentos Relacionados
- Quality of coffee (Coffea arabica L.) pre-processed in coconut
- AdubaÃÃo lÃquida na implantaÃÃo da lavoura cafeeira (Coffea arabica L.)
- Comportamento de progÃnies de cafeeiro (Coffea arabica L.) em Varginha-MG
- Perfomance of coffee plant (Coffea arabica L.) to drip irrigation levels
- Avaliação de diferentes métodos de secagem do café (coffea arabica l.) cereja descascado