Influence of histone H1 on the in vitro replication of DNA and chromatin.
AUTOR(ES)
Halmer, L
RESUMO
The influence of histone H1 on DNA replication was studied using the SV40 in vitro replication system with two different templates: histone H1/DNA complexes and SV40 minichromosomes reconstituted with H1. We found that the cytosolic extracts used as a source of enzymes for in vitro replication contained high amounts of RNA which competed with template DNA for the binding of histone H1. Removal of this RNA was necessary to ensure the stability of the templates thus allowing for the first time the study of the replication of histone H1-carrying templates in vitro. In contrast to the inhibitory effect of histone H1 on the initiation of transcription, bound H1, when present at physiological ratios, does not interfere with the in vitro replication of DNA and minichromosomes. Ratios higher than one H1 molecule per nucleosome affected replication of reconstituted SV40 minichromosomes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=306758Documentos Relacionados
- Histone H1 deposition and histone-DNA interactions in replicating chromatin.
- Influence of histone acetylation on the solubility, H1 content and DNase I sensitivity of newly assembled chromatin.
- Prothymosin alpha modulates the interaction of histone H1 with chromatin.
- Effects of cell cycle dependent histone H1 phosphorylation on chromatin structure and chromatin replication.
- A lysine-rich protein functions as an H1 histone in Dictyostelium discoideum chromatin.
